Efficiency of essential oils to control Colletotrichum theobromicola in vitro

Abstract

Essential oils promote the inhibitory control of several fungi, including those within the genus Colletotrichum, the causal agent of Anthracnose, a disease which may occur at any stage of development in various crops, reducing up to 70% of crop production in some cases. Thus, the use of alternative products constitutes an important strategy for the integrated management, promoting less persistent molecules in the environment and lower toxicity rates, providing health benefits to producers and consumers of agricultural products. In this context, the present study evaluated the in vitro fungitoxic effect of essential oils from Java citronella (Cymbopogon winterianus), clove (Syzygium aromaticum), eucalyptus (Eucalyptus globulus) and rose pepper (Schinus terebinthifolius) on the mycelial growth of Colletotrichum theobromicola. The experiment was performed under completely randomized design, in a 4x5 factorial scheme (4 essential oils x 5 concentrations), with five replications, and the experimental unit consisting of a Petri dish. The treatments were generated by combining the concentrations (0, 5, 10, 15, 25 and 50 μL mL-1) of essential oils (citronella, clove, eucalyptus and rose pepper). The plates were inoculated with the pathogen C. theobromicola and incubated for seven days at 25 ± 2 °C. To verify the difference between treatments, the percentage of mycelial growth inhibition (PGI) was estimated. The mycelial growth of C. theobromicola was significantly reduced with increasing concentrations of essential oils. At a concentration of 50 μL mL-1, the essential oil from S. terebinthifolius showed the best result inhibiting 54.57% of mycelial growth, followed by the oil from S. aromaticum (49.26%), C. winterianus (23.70%) and E. globulus (17.90%). All the studied oils showed antifungal activity.