Phenotypic diversity of bacteria in root nodules of Dalbergia ecastaphyllum (L.) Taub. (Fabaceae)

Authors

DOI:

https://doi.org/10.18227/1982-8470ragro.v15i0.6920

Keywords:

Legumes. Symbiotic relationship. Rhizobia.

Abstract

Many species belonging to family Fabaceae are able to establish symbiotic relationships with nitrogen-fixing bacteria. Studies developed with Dalbergia ecastaphyllum (L.) Taub., for example, have demonstrated the symbiotic potential of this species. In this sense, this study aimed to analyze the phenotypic characteristics of rhizobia isolated from D. ecastaphyllum and to identify whether these bacterial isolates are capable of establishing symbiotic relationships with Vigna unguiculata (L.) Walp. D. ecastaphyllum seeds were sown in soil samples collected in Japaratinga, Alagoas, Brazil, in three zones located at different distances from the high tide line. At 60 days after the emergence of the plants, nodulation in the roots of D. ecastaphyllum was analyzed and the bacteria were isolated. Subsequently, the phenotypic characterization of the bacteria was carried out based on some criteria (growth time, type of pH, colony color, type of mucus and amount of mucus). For the nodulation test, the species V. unguiculata was used. In total, 17 phenotypic types of bacterial isolates were identified, of which 8 are fast-growing and 9 are intermediate-growth. Regarding the type of pH, 7 phenotypes are acidic pH, 8 are neutral pH and 2 are alkaline pH. As for the coloring of the bacterial colonies, 5 are yellow, 7 white and 5 pink. It was also observed the presence of consistent and aqueous mucus in colonies from the three different areas. Only 7 bacterial phenotypic types and the commercial inoculant were efficient in the nodulation of V. unguiculata.

Author Biographies

Lidia Del Carmen Trujillo Pacheco, Universidade Federal de Alagoas

Universidade Federal de Alagoas, Centro das Engenharias e Ciências Agrárias, Programa de Pós-Graduação em Agronomia - Produção Vegetal.

Jônatas Oliveira Costa, Universidade Federal de Alagoas

Universidade Federal de Alagoas, Instituto de Ciências Biológicas e da Saúde.

Flávia de Barros Prado Moura, Universidade Federal de Alagoas

Universidade Federal de Alagoas, Instituto de Ciências Biológicas e da Saúde, Programa de Pós-Graduação em Agronomia - Produção Vegetal.

José Jailson Lima Bezerra, Universidade Federal de Pernambuco

Doutorando pelo Programa de Pós-Graduação em Biologia Vegetal, Departamento de Botânica, Universidade Federal de Pernambuco.

Ana Paula do Nascimento Prata, Universidade Federal de Alagoas

Universidade Federal de Alagoas, Centro das Engenharias e Ciências Agrárias, Programa de Pós-Graduação em Agronomia - Produção Vegetal.

Published

20/04/2021

Issue

Section

Original Scientific Article